OUR WORK

Our Discoveries

Pioneering Field Techniques for Cotton-top Tamarin Monitoring

Cotton-top tamarins are small, agile primates that live high in the dense forest canopy, making them difficult to observe and study. Traditional wildlife monitoring methods were not adequate for collecting detailed information about their behavior, social organization, and habitat use. To overcome these challenges, Proyecto Tití scientists developed innovative techniques that revolutionized the study of this elusive and critically endangered species in Colombia’s tropical forests. These methods have since become foundational tools in primate field research and continue to guide conservation efforts today.

Cotton-top tamarin Proyecto Titi Michael Ryan-Clark
Marking for Identification Field-Research Cotton-top tamarin Proyecto Titi Suzi Eszterhas
In tree with transmitter

Developing Safe and Effective Monitoring Techniques

Because cotton-top tamarins are not sexually dimorphic and lack distinct physical features that easily differentiate individuals, Proyecto Tití’s scientists needed creative approaches to identify and track them safely in the wild.

  • Individual Identification: Small amounts of commercially available, non-toxic hair dye were applied to the tamarins’ white fur—on the head, arms, legs, or chest—to allow researchers to recognize individuals from a distance without causing harm or stress.
  • Radio Telemetry: A lightweight radio transmitter was attached to an adult male in each study group using a specially designed backpack-style harness. This allowed researchers to precisely track group movements, monitor habitat use, and document social interactions.

Impact on Conservation and Research

These pioneering methods made it possible to collect comprehensive, long-term data on the cotton-top tamarin’s social structure, behavior, diet, reproductive biology, and habitat use. The insights gained have been instrumental in shaping data-driven conservation strategies, guiding habitat restoration, informing education programs, and contributing to international assessments such as the IUCN Red List.

Savage, A., Giraldo, L.H., Blumer, E.S, Soto, L.H., Burger, W.T., Snowdon, C.T. 1993. Field techniques for monitoring cotton-top tamarin (Saguinus oedipus oedipus) in Colombia. American Journal of Primatology. 31:189-196. https://doi.org/10.1002/ajp.1350310304

Savage, A., Giraldo, H., Soto, L.H., Garcia, F.E., Nassar, F. 2003. Proyecto Tití: Establecimiento de técnicas de campo para el monitoreo a largo plazo del Tití Cabeciblanco (Saguinus oedipus) en Colombia. In: Primatologia del Nuevo Mundo, Eds F. Nassar, V. Pereriam & A. Savage, pp. 40-46.

Monitoring Cotton-top Tamarin Populations Through Innovative Techniques

For many years, no one knew exactly how many cotton-top tamarins remained in the wild. These tiny primates live high in dense forest canopies and are quick to flee or fall silent when humans approach, making them extremely difficult to count. To overcome this challenge, Proyecto Tití partnered with Dr. Len Thomas of the University of St. Andrews to create a groundbreaking method for estimating the cotton-top tamarin population. The team played recordings of cotton-top tamarin calls during systematic forest walks, prompting the curious monkeys to respond and reveal their locations—a clever technique known as the “lure strip transect” survey. This innovation revolutionized how scientists monitor this elusive species in its natural habitat.

Using this approach, Proyecto Tití conducted the first comprehensive population survey of cotton-top tamarins in the mid-2000s. The results were sobering: only about 7,000 individuals were estimated to remain in the wild across Colombia—a steep decline from the tens of thousands that once existed in the 1960s before deforestation, the pet trade, and research capture decimated their numbers. Most surviving tamarins were found in small, isolated forest fragments. This discovery underscored the urgent need to protect these last remaining forests and prevent further habitat loss. The study helped elevate the species’ global conservation status, positioning the cotton-top tamarin as one of the world’s most endangered primates, and galvanized national and international action to safeguard it.

Several years later, Proyecto Tití and Dr. Thomas repeated the survey to assess the species’ progress after years of focused conservation work. The findings brought cautious optimism: the population appeared stable, showing no significant declines. This stability reflected the positive impact of ongoing conservation actions—such as forest protection and restoration, community education, and efforts to reduce the illegal pet trade—carried out by Proyecto Tití, the Colombian government, and partner organizations. However, the study also made clear that the species remains highly vulnerable, with low numbers and persistent threats from habitat loss and human encroachment.

Impact on Conservation

Being able to accurately estimate cotton-top tamarin numbers gave Proyecto Tití a vital scientific baseline for measuring progress and guiding strategy. Knowing how many tamarins remain—and where they survive—allows researchers to monitor trends over time, identify priority areas for protection, and detect early warning signs of decline.

This data-driven approach has become central to Proyecto Tití’s conservation planning, helping ensure that every action—from reforestation to community engagement—is grounded in science. The relatively stable population trends offer hope, demonstrating that dedicated conservation efforts can make a measurable difference. At the same time, the surveys serve as a critical monitoring tool: any future population drops would signal the need to intensify protection.

Thanks to this pioneering collaboration and innovative counting technique, Proyecto Tití now has a far clearer understanding of the cotton-top tamarin’s status—knowledge that continues to strengthen and inspire conservation efforts to secure this species’ future.

Miller, L., Savage, A., Giraldo, H. 2004. Quantifying the remaining forested habitat within the historic distribution of the cotton-top tamarin (Saguinus oedipus) in Colombia: Implications for long-term conservation. American Journal of Primatology, 64:451-457. https://doi.org/10.1002/ajp.20091

Ruiz Miranda, C.R., Savage, A., Forero, F. 2023. Aplicaciones del monitoreo acústico activo y pasivo para conservación deNew World Primates. Neotropical Primates 29(1) 1-13.

Savage, A., Thomas, L., Feilen, K.L., Soto, L.H., Pearson, M., Medina, F.S., Emeris, G., Guillen, R.R. 2016. An assessment of the population of cotton-top tamarins (Saguinus oedipus) and their habitat in Colombia. PLoS One 11 (12) e0168324.
https://doi.org/10.1371/journal.pone.0168324

Savage, A., Thomas, L., Leighty, K.A., Soto, L.H., Medina, F.S. 2010. Primate on the brink: novel survey method finds dramatic decline of wild cotton-top tamarin population. Nature Communications 1, 30. https://doi.org/10.1038/ncomms1030

Savage, A., Thomas, L., Soto, L., Causado, J. 2010. Investigacíon preliminar de la población del titi cabeciblando (Saguinus oedipus) en el Noroccidente Colombiano (Departamentos de Atlántico y Bolívar). In: Primatología en Colombia: Avances al Princípio del Milénio. Eds: Pereria-Bengoa, V., Stevenson, P.R., Bueno, M.L, Nassar-Montoya, F., Fundación Universitaria San Martin, Bogotá, Colombia, pp. 99-109.

Savage, A., Soto, L., Lamilla, I. and Guillen, R. 2009. Cotton-top tamarin Saguinus oedipus (Linnaeus, 1758). In: R. A. Mittermeier et al., Primates in Peril: The World’s 25 Most Endangered Primates 2008–2010, pp.68-71. IUCN/SSC Primate Specialist Group (PSG), International Primatological Society (IPS), and Conservation International (CI), Arlington, VA.

Understanding the Reproductive Biology of Cotton-top Tamarins

Proyecto Tití’s long-term research has greatly advanced our understanding of cotton-top tamarin reproductive biology. Through non-invasive techniques and decades of field studies, we’ve revealed key aspects of their breeding strategies—insights that are essential for effective conservation planning.

Non-Invasive Hormonal Monitoring

Proyecto Tití uses non-invasive hormone analysis, examining fecal samples to assess hormonal concentrations. This approach allows scientists to evaluate how factors like social organization, environmental change, and habitat disturbance influence reproduction. These data guide strategies to promote reproductive health in wild populations.

Seasonal Breeding Patterns

Cotton-top tamarins breed seasonally, with births occurring primarily during the wet season, when food is most abundant. This timing ensures that mothers have the nutrition needed to support lactation and infant care, increasing infant survival rates. Understanding these patterns is critical for predicting how a changing climate may affect reproduction and offspring success in the future.

Social Structure and Reproductive Roles

In cotton-top tamarin groups, typically only the dominant female reproduces, while subordinate females experience reproductive suppression. However, shifts in group structure—such as the loss of a dominant individual—can alter reproductive opportunities for others. Recognizing these dynamics helps scientists predict population changes and manage group compositions in both wild and managed care settings.

By integrating these reproductive insights into conservation planning, Proyecto Tití strengthens efforts to protect and sustain cotton-top tamarin populations in their natural habitats, ensuring the long-term survival of this critically endangered species.

Lasley, B.L., Savage, A. Reproductive Technology: Advances in the Understanding of Primate Reproductive Endocrinology. 2006. In: Perspective in Primatology, Ed. C. Campbell, A. Fuentes, K.C. Mackinnon, M. Panger, & S.K. Beader, Oxford University Press, New York, NY pp. 356-369.

Savage, A., Shideler, S.E., Soto, L.H., Causado, J.C., L. Giraldo, L.H., Lasley, B.L., Snowdon, C.T. 1997. Reproductive events of wild cotton-top tamarins (Saguinus oedipus) in Colombia. American Journal of Primatology, 43:329-337. https://doi.org/10.1002/(SICI)1098-2345(1997)43:4<329::AID-AJP4>3.0.CO;2-Z

Wheaton, C.J., Feilen, K.L., Soto, L.H., Medina, F., Emeris, G., Guillen, R., Savage, A. 2022. Seasonality of reproduction in wild cotton-top tamarins (Saguinus oedipus) in Colombia. American Journal of Primatology, 84(2): e23359 https://doi.org/10.1002/ajp.23359

Wheaton CJ, Savage A, Soto L, Medina F, Emeris G, Guillen R. 2012. Tolerance may lead to loss of tenure in tamarin societies: Use of non-invasive faecal reproductive steroid monitoring and behaviour observations to reveal reproductive and life-history traits in wild female cotton-top tamarins (Saguinus oedipus) in Colombia. Wien Tierärztl Monat 99 (Suppl 1): 16.

Wheaton, C. J., Savage, A., and B. L. Lasley, 2010. Advances in the understanding of primate reproductive endocrinology. In Primates in Perspective, 2nd Edition, Campbell, C. J., Fuentes, A., MacKinnon, K. C., Bearder, S. K. and R. M. Stumpf (Eds.). Oxford University Press, Inc. New York, New York. Pg. 377-389.

Genetic Insights into Cotton-top Tamarins

Proyecto Tití’s research into the genetics of the cotton-top tamarin has revealed crucial insights into the species’ genetic diversity, population structure, and resilience. These findings play a vital role in guiding conservation strategies for this critically endangered primate.

Historical Genetic Diversity and Population Structure

In collaboration with Dr. Linett Rasmussen from Copenhagen Zoo and Dr. Iván Soto from the Universidad de Antioquia, Proyecto Tití conducted a comprehensive genetic study combining historical museum specimens with modern wild samples. Using DNA extracted from hair samples, the results showed that cotton-top tamarins once existed in two genetically distinct populations, likely separated by Colombia’s Paramillo Massif mountain range. This work established a genetic baseline from before major human-driven environmental changes, providing a reference point to assess how habitat loss and fragmentation have affected the species’ genetic health over time.

Current Genetic Challenges

Recent analyses indicate a decline in genetic diversity, especially within northeastern populations. Evidence of inbreeding, such as reduced heterozygosity and long regions of homozygosity, reflects the severe population declines caused by deforestation and the mass exportation of cotton-top tamarins for biomedical research during the 1960s. Encouragingly, despite this genetic bottleneck, our studies show no significant increase in harmful mutations, suggesting that the species has retained some level of genetic resilience.

Immune System Genetic Variability

Research into the major histocompatibility complex (MHC) genes—key components of immune system function—has revealed that cotton-top tamarins have limited MHC class I variability. This reduced diversity could compromise their ability to fight diseases and adapt to new environmental challenges, underscoring the importance of maintaining and restoring healthy, genetically diverse populations.

Conservation Implications

Understanding the genetic makeup of cotton-top tamarins is essential for their conservation. Insights into population structure and genetic diversity inform habitat protection priorities, inform potential translocation of trafficked cotton-top tamarins, and help predict how populations might respond to environmental pressures. By examining genetic diversity, Proyecto Tití can monitor the resiliency of cotton-top tamarins to changes in their environment.

Gyllensten, U., Bergstrom, T., Joseffsson, A., Sundvall, M., Savage, A., Giraldo, L.H., Blumer, E.S., Watkins, D.I. 1994. The cotton-top tamarin revisted: Limited Mhc Class I polymorphism of wild tamarins and limited nucleotide diversity of the class II DQA1, DQB1 and DRB Loci. Immunogenetics, 40(3) 167-176.

Rasmussen, L. Fontsere, C., Soto-Calderon, I.D., Guillen, R., Savage, A., Hansen, A.J., Hvilsom, C., Gilbert, T.P. 2023. Assessing the genetic composition of cotton-top tamarins (Saguinus oedipus) before sweeping anthropogenic impact. Molecular Genetics 32(20):5514-5527.

Understanding the Diet of Cotton-top Tamarins

Over more than two decades of research, Proyecto Tití has gained valuable insights into the dietary habits of cotton-top tamarins, revealing how seasonal changes and climate variability shape their feeding behavior and resource use.

Seasonal Dietary Patterns

Cotton-top tamarins show clear seasonal variations in their diet, closely tied to food availability. During the wet season, when fruits are plentiful, they feed mainly on fruits and insects. In the dry season, when fruits are scarce, they shift to consuming more plant exudates—such as gums and saps—and increase their intake of insects to meet their nutritional needs.

Adaptability to Environmental Changes

This ability to adjust their diet reflects the tamarins’ remarkable flexibility in coping with seasonal shifts. However, their adaptability has limits. Prolonged dry periods, deforestation, and habitat degradation can drastically reduce food availability, posing serious challenges to their health, reproduction, and long-term survival.

Conservation Implications

A deep understanding of the tamarins’ dietary ecology is vital for effective habitat management and restoration. Conservation efforts must ensure the protection and regeneration of forests that provide essential seasonal food resources. Additionally, strategies to mitigate the impacts of a changing climate and environmental disruption are key to safeguarding the future of this critically endangered species.

Savage, A., Snowdon, C.T., Soto, L., Castro, J., Medina, F., Emeris, G., Garcias, L.C., Guillen, R. 2022. The impacts of seasonal variation and climate on food utilization in a population of critically endangered cotton-top tamarins (Saguinus oedipus) in Colombia: A 22 year longitudinal study. American Journal of Primatology, DOI: 10.1002/ajp.23415

Infant Development and Care in Wild Cotton-top Tamarins

In the wild, cotton-top tamarins depend on a cooperative breeding system, where the entire group contributes to raising the young. Proyecto Tití’s decades of field research have revealed how this teamwork is essential not only for infant survival but also for maintaining the social bonds that define tamarin life.

Birth and Early Development

Cotton-top tamarins typically give birth to twins, though singletons and triplets occasionally occur. Newborns are highly dependent and require near-constant care. During the first few weeks of life, infants are carried almost continuously—primarily by the father and other group members—while the mother carries them mainly for nursing and resting. This close physical contact strengthens social bonds and provides warmth, protection, and comfort during the most vulnerable stage of life.

Roles of Caregivers

Infant care in tamarins is learned, not instinctive. Young group members gain valuable experience by helping care for their siblings, preparing them to be successful parents in the future. While mothers nurse the infants, fathers play a leading role in carrying them, and older siblings assist with carrying, grooming, and sharing food. This shared caregiving reduces the physical burden on parents and increases the infants’ chances of survival. Group members also take turns keeping watch for predators while others care for the young, reflecting a high level of cooperation and vigilance within the group.

Weaning and Independence

As infants grow, they begin spending more time off their caregivers, exploring their surroundings, and trying solid foods. Weaning generally occurs between 10 and 18 weeks of age, depending on environmental conditions and group dynamics. During this critical period, young tamarins learn essential survival and social skills through observation and interaction with their family group.

Factors Influencing Infant Survival

Proyecto Tití’s long-term studies show that infant survival depends on several factors, including litter size, infant caretaking experience, and number of caregivers. Single infants often have higher survival rates than twins or triplets due to reduced competition for care and resources. Groups with experienced caregivers are more successful in raising young, underscoring the importance of stable social structures in wild populations.

Understanding the complexities of infant development and cooperative care is critical for cotton-top tamarin conservation. By recognizing the vital role of social organization and environmental conditions, Proyecto Tití can design conservation strategies that support the natural behaviors essential to the species’ survival.

Savage, A., Giraldo, H.G., Soto, L.H., Snowdon, C.T. 1996. Demography, group composition and dispersal in wild cotton-top tamarins. Guest Editors A. Savage & A.J. Baker, American Journal of Primatology, 38(1), 85-100. https://doi.org/10.1002/(SICI)1098-2345(1996)38:1<85::AID-AJP7>3.0.CO;2-P

Social Structure, Group Composition, and Dispersal in Wild Cotton-top Tamarins

Understanding the social organization, group dynamics, and dispersal patterns of cotton-top tamarins is essential for developing effective conservation strategies. These aspects of tamarin life directly influence population stability, genetic diversity, and reproductive success.

Group Composition and Social Hierarchy

In the wild, cotton-top tamarins typically live in small, cohesive groups ranging from 2 to 13 individuals, with an average of about seven members in each group. Each group usually includes a dominant breeding pair and their offspring from multiple years. The dominant female often suppresses reproduction in subordinate females through behavioral cues and possibly chemical signals, ensuring that only she breeds within the group.

This hierarchical structure supports a cooperative breeding system, where non-breeding individuals assist with infant care, food sharing, and vigilance. Such cooperation strengthens social bonds and enhances the survival of both offspring and the group as a whole.

Dispersal Patterns and Group Dynamics

Dispersal—the movement of individuals away from their birth group—is a key process in tamarin society. Both males and females typically leave their natal group upon reaching sexual maturity (approximately 12 to 18 months of age). Dispersal helps prevent inbreeding and allows individuals to seek new breeding opportunities.

However, dispersal also involves significant risks: solitary tamarins are more vulnerable to predators and may struggle to find or integrate into a new group. Group stability is influenced by factors such as the availability of suitable forest habitat and the presence of potential mates. In fragmented landscapes, restricted movement between forest patches can limit dispersal, reducing opportunities for group formation and impacting genetic diversity.

Implications for Conservation

Knowledge of tamarin social systems and dispersal behavior is vital for conservation planning and modeling future population outcomes. Protecting and restoring continuous forest corridors allows for natural dispersal and group formation, supporting genetic exchange and long-term population viability.

Savage, A., Snowdon, C.T., Soto, L., Medina, F., Emeris, G., Guillen, R. Factors influencing the survival of wild cotton-top tamarin (Saguinus oedipus) infants. 2021. American Journal of Primatology, 83(7) e23262 https://doi.org/10.1002/ajp.23262

Savage, A., Soto, L., Medina, F., Emeris, G., Soltis, J. 2009. Litter size and infant survivorship in wild groups of cotton-top tamarins (Saguinus oedipus) in Colombia. American Journal of Primatology 71:1-5. https://doi.org/10.1002/ajp.20696

Savage, A., Snowdon, C.T., Giraldo, H.L., Soto, L.H. 1996. Parental care patterns and vigilance in wild cotton-top tamarins (Saguinus oedipus). In: Adaptive Radiations of Neotropical Primates. Eds. M. Norconk, A. Rosenberger, P. Garber, Plenum Press, New York, NY, pp.197-199.

Ziegler, T.E., Tecot, S.R., Fernández-Duque, E., Savage, A., Snowdon, C.T., 2022. Nonhuman primate paternal care: Species and individual differences in behavior and mechanisms. In: Patterns of Parental Behavior, Ed. G. Gonzáles-Mariscal, Springer, https://doi.org/10.1007/978-3-030-97762-7_7

Health and Well-being of Wild Cotton-top Tamarins

Proyecto Tití’s extensive research on the health of wild cotton-top tamarins has yielded important insights into their physiological condition, parasite prevalence, and nutritional status. These findings are essential for guiding conservation actions that support the long-term survival of this critically endangered species.

Overall Health Assessments

Field evaluations show that wild cotton-top tamarins generally maintain good health in their natural habitats. Clinical examinations reveal that most individuals exhibit normal physical conditions, with little evidence of disease or malnutrition. These results emphasize the importance of preserving intact forest environments, which provide the resources necessary for maintaining healthy populations.

Parasitic Infections

Studies have identified several gastrointestinal parasites, including protozoa and helminths, present in wild tamarin populations. Although these parasites are relatively common, they rarely cause overt illness. Continuous health monitoring remains important, however, as environmental changes—such as habitat disturbance, proximity to domestic animals/humans, or altered food availability—could increase parasite transmission or severity, potentially affecting population health.


Vitamin D Status

Studies on vitamin D levels in wild cotton-top tamarins show that these primates maintain adequate concentrations of this vital nutrient through natural sunlight exposure. Vitamin D plays a key role in bone development and calcium metabolism, highlighting the importance of access to sunlight in both wild habitats and managed care settings.

Understanding the health dynamics of wild cotton-top tamarins is critical for effective conservation planning. Protecting their natural habitats, monitoring key health indicators, and mitigating environmental threats all contribute to sustaining resilient and thriving tamarin populations.

Barrera, D., Pereira-Bengoa, V., Nassar-Montoya, F., Savage, A., Soto, L., Giraldo, H., Garcia, F., Ramirez, O.C. 2010. Parásitos en una población natural de tití cabeza blanca (Saguinus oedipus), Hacienda El Ceibal, Colombia. In: Primatología en Colombia: Avances al Princípio del Milénio. Eds: Pereria-Bengoa, V., Stevenson, P.R., Bueno, M.L, Nassar-Montoya, F., Fundación Universitaria San Martin, Bogotá, Colombia, pp. 161-169.

Nassar-Montoya, F., Pereira-Bengoa, V., Savage, A., Soto, L., Giraldo, H., Garcia, F., Ramirez, O.C. 2010. Evaluación de la salud de uma población natural de tití cabeza blanca (Saguinus oedipus), Hacienda El Ceibal. In: Primatología en Colombia: Avances al Princípio del Milénio. Eds: Pereria-Bengoa, V., Stevenson, P.R., Bueno, M.L, Nassar-Montoya, F., Fundación Universitaria San Martin, Bogotá, Colombia, pp. 147-160.

Power, M.L., Oftedal, O.T., Savage, A., Blumer, E.S., Soto, L.H., Chen, T.C., Holick, M.F. 1997. Assessing Vitamin Dstatus of callitrichids: Baseline data from wild cotton-top tamarins (Saguinus oedipus) in Colombia. Zoo Biology, 16:39-46. https://doi.org/10.1002/(SICI)1098-2361(1997)16:1<39::AID-ZOO6>3.0.CO;2-C

Our Approach to Conservation

Proyecto Tití: An Integrated Approach to Cotton-top Tamarin Conservation

Proyecto Tití is a pioneering conservation initiative dedicated to safeguarding the critically endangered cotton-top tamarin. We recognize that lasting conservation success depends on more than scientific research—it requires the active involvement of people and communities who share the tamarins’ forest home.

Our comprehensive approach combines long-term field research, habitat protection and restoration, community engagement, sustainable livelihood creation, and environmental education to secure a future for this remarkable species and the ecosystems it depends on.

Savage, A., Guillen, R., Vega, J., Soto, L. 2013. El Proyecto Tití: Medidas Efectivas para la Conservacion del Tití Cabeciblanco (Saguinus oedipus). In: Defler TR, Stevenson PR, Bueno MT & Guzmán-Caro DC. Primates Colombianos en Peligro de Extinción. Asociación Primatológica Colombiana, Bogotá, p 23-38.

Savage, A. & Guillen, R. 2012. Conserving cotton-top tamarins (Saguinus oedipus) through effective captive management, public engagement and in situ conservation efforts. International Zoo Yearbook, 46(1) 56-70. https://doi.org/10.1111/j.1748-1090.2011.00159.x

Savage, A., Guillen, R., Lamilla, I., Soto, L. 2010. Developing an effective conservation program for cotton-top tamarins (Saguinus oedipus) in Colombia that incorporates strategies addressing integrated conservation and community development initiatives. American Journal of Primatology, 72(5):379-390. https://doi.org/10.1002/ajp.20770

Savage, A., Giraldo, L.H., Soto, L.H., Garica, F. 2000. Proyecto Tití: Esfuerzos Integrados para la Conservación del Tití Cabeciblanco (Saguinus aedipus) en Colombia. Actitudes Hacia La Fuana En LatinoAmérica, Humane Society International, Washington, DC, pp. 221-236.

Savage, A., Soto, L.H., Giraldo, L.H. Proyecto Titi: 2000. The conservation impact of long-term monitoring of the endangered cotton-top tamarin population on local communities. Proceedings of the Second International Conference on Wildlife Rehabilitation. Humane Society International, pp. 24-34.